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ecotropic gp80  (Addgene inc)


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    Structured Review

    Addgene inc ecotropic gp80
    A. Diagram of EcoHIV reporter vector containing enhanced firefly luciferase (eLuc) derived from human HIV-1NL4-3 vector. The eLuc gene was inserted between Env and Nef with a self-cleaving 2A peptide before Nef, while the gp120 of HIV-1 was replaced with <t>gp80</t> from the <t>ecotropic</t> murine leukemia virus. B. Selected gRNAs targeting HIV-1 LTR, Gag and Pol regions. The seed sequences targeting 400 bp within U3 region with red/underlined PAM at the sense strand and black/underlined PAM at the antisense strand. Most of them can be also paired for Cas9 nickase and RNA-guided FokI nuclease. The TATA box and transcriptional factor binding motifs are shadowed and labeled in green. C. Lentiviral reporter vectors for spCas9 and gRNA cloning.
    Ecotropic Gp80, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecotropic+gp80/pHCMV-EcoEnv+(Plasmid+%2315802)/pmc04851589-122-21-25
    Average 93 stars, based on 35 article reviews
    ecotropic gp80 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS"

    Article Title: Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS

    Journal: AIDS (London, England)

    doi: 10.1097/QAD.0000000000001079

    A. Diagram of EcoHIV reporter vector containing enhanced firefly luciferase (eLuc) derived from human HIV-1NL4-3 vector. The eLuc gene was inserted between Env and Nef with a self-cleaving 2A peptide before Nef, while the gp120 of HIV-1 was replaced with gp80 from the ecotropic murine leukemia virus. B. Selected gRNAs targeting HIV-1 LTR, Gag and Pol regions. The seed sequences targeting 400 bp within U3 region with red/underlined PAM at the sense strand and black/underlined PAM at the antisense strand. Most of them can be also paired for Cas9 nickase and RNA-guided FokI nuclease. The TATA box and transcriptional factor binding motifs are shadowed and labeled in green. C. Lentiviral reporter vectors for spCas9 and gRNA cloning.
    Figure Legend Snippet: A. Diagram of EcoHIV reporter vector containing enhanced firefly luciferase (eLuc) derived from human HIV-1NL4-3 vector. The eLuc gene was inserted between Env and Nef with a self-cleaving 2A peptide before Nef, while the gp120 of HIV-1 was replaced with gp80 from the ecotropic murine leukemia virus. B. Selected gRNAs targeting HIV-1 LTR, Gag and Pol regions. The seed sequences targeting 400 bp within U3 region with red/underlined PAM at the sense strand and black/underlined PAM at the antisense strand. Most of them can be also paired for Cas9 nickase and RNA-guided FokI nuclease. The TATA box and transcriptional factor binding motifs are shadowed and labeled in green. C. Lentiviral reporter vectors for spCas9 and gRNA cloning.

    Techniques Used: Plasmid Preparation, Luciferase, Derivative Assay, Virus, Binding Assay, Labeling, Cloning

    Related Articles

    Luciferase:

    Article Title: Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS
    Article Snippet: .. The pNL4-3-EcoHIV- firefly -luciferase (eLuc) vector ( ) was then generated by replacing the coding region of gp120 Env with the ecotropic gp80 from pHCMV-EcoEnv (Addgene plasmid #15802, a gift from Dr. Miguel Sena-Esteves)[ 34 ]. ..

    Plasmid Preparation:

    Article Title: Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS
    Article Snippet: .. The pNL4-3-EcoHIV- firefly -luciferase (eLuc) vector ( ) was then generated by replacing the coding region of gp120 Env with the ecotropic gp80 from pHCMV-EcoEnv (Addgene plasmid #15802, a gift from Dr. Miguel Sena-Esteves)[ 34 ]. ..

    Article Title: Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS
    Article Snippet: .. The pNL4-3-EcoHIV-firefly-luciferase (eLuc) vector (Fig. 1A) was then generated by replacing the coding region of gp120 Env with the ecotropic gp80 from pHCMVEcoEnv (Addgene plasmid #15802, a gift from Dr. Miguel Sena-Esteves)[34]. ..

    Generated:

    Article Title: Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS
    Article Snippet: .. The pNL4-3-EcoHIV- firefly -luciferase (eLuc) vector ( ) was then generated by replacing the coding region of gp120 Env with the ecotropic gp80 from pHCMV-EcoEnv (Addgene plasmid #15802, a gift from Dr. Miguel Sena-Esteves)[ 34 ]. ..

    Article Title: Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS
    Article Snippet: .. The pNL4-3-EcoHIV-firefly-luciferase (eLuc) vector (Fig. 1A) was then generated by replacing the coding region of gp120 Env with the ecotropic gp80 from pHCMVEcoEnv (Addgene plasmid #15802, a gift from Dr. Miguel Sena-Esteves)[34]. ..



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    A. Diagram of EcoHIV reporter vector containing enhanced firefly luciferase (eLuc) derived from human HIV-1NL4-3 vector. The eLuc gene was inserted between Env and Nef with a self-cleaving 2A peptide before Nef, while the gp120 of HIV-1 was replaced with <t>gp80</t> from the <t>ecotropic</t> murine leukemia virus. B. Selected gRNAs targeting HIV-1 LTR, Gag and Pol regions. The seed sequences targeting 400 bp within U3 region with red/underlined PAM at the sense strand and black/underlined PAM at the antisense strand. Most of them can be also paired for Cas9 nickase and RNA-guided FokI nuclease. The TATA box and transcriptional factor binding motifs are shadowed and labeled in green. C. Lentiviral reporter vectors for spCas9 and gRNA cloning.
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    A. Diagram of EcoHIV reporter vector containing enhanced firefly luciferase (eLuc) derived from human HIV-1NL4-3 vector. The eLuc gene was inserted between Env and Nef with a self-cleaving 2A peptide before Nef, while the gp120 of HIV-1 was replaced with <t>gp80</t> from the <t>ecotropic</t> murine leukemia virus. B. Selected gRNAs targeting HIV-1 LTR, Gag and Pol regions. The seed sequences targeting 400 bp within U3 region with red/underlined PAM at the sense strand and black/underlined PAM at the antisense strand. Most of them can be also paired for Cas9 nickase and RNA-guided FokI nuclease. The TATA box and transcriptional factor binding motifs are shadowed and labeled in green. C. Lentiviral reporter vectors for spCas9 and gRNA cloning.
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    Image Search Results


    A. Diagram of EcoHIV reporter vector containing enhanced firefly luciferase (eLuc) derived from human HIV-1NL4-3 vector. The eLuc gene was inserted between Env and Nef with a self-cleaving 2A peptide before Nef, while the gp120 of HIV-1 was replaced with gp80 from the ecotropic murine leukemia virus. B. Selected gRNAs targeting HIV-1 LTR, Gag and Pol regions. The seed sequences targeting 400 bp within U3 region with red/underlined PAM at the sense strand and black/underlined PAM at the antisense strand. Most of them can be also paired for Cas9 nickase and RNA-guided FokI nuclease. The TATA box and transcriptional factor binding motifs are shadowed and labeled in green. C. Lentiviral reporter vectors for spCas9 and gRNA cloning.

    Journal: AIDS (London, England)

    Article Title: Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS

    doi: 10.1097/QAD.0000000000001079

    Figure Lengend Snippet: A. Diagram of EcoHIV reporter vector containing enhanced firefly luciferase (eLuc) derived from human HIV-1NL4-3 vector. The eLuc gene was inserted between Env and Nef with a self-cleaving 2A peptide before Nef, while the gp120 of HIV-1 was replaced with gp80 from the ecotropic murine leukemia virus. B. Selected gRNAs targeting HIV-1 LTR, Gag and Pol regions. The seed sequences targeting 400 bp within U3 region with red/underlined PAM at the sense strand and black/underlined PAM at the antisense strand. Most of them can be also paired for Cas9 nickase and RNA-guided FokI nuclease. The TATA box and transcriptional factor binding motifs are shadowed and labeled in green. C. Lentiviral reporter vectors for spCas9 and gRNA cloning.

    Article Snippet: The pNL4-3-EcoHIV- firefly -luciferase (eLuc) vector ( ) was then generated by replacing the coding region of gp120 Env with the ecotropic gp80 from pHCMV-EcoEnv (Addgene plasmid #15802, a gift from Dr. Miguel Sena-Esteves)[ 34 ].

    Techniques: Plasmid Preparation, Luciferase, Derivative Assay, Virus, Binding Assay, Labeling, Cloning